Richard M. Van Rijn

In my laboratory we use innovative strategies and techniques to provide a better understanding of the molecular mechanism by which G-protein coupled receptors (GPCRs) function, propagate signal transduction and modulate behavior. GPCRs form the largest protein family in the human genome and are crucial in relaying extracellular information into the cell. GPCRs are involved in many diverse physiological responses, including light perception, taste, immune responses, cardiovascular activity and neurotransmission. Currently, 30-40% of all drugs, approved by the Federal Drug Administration, target GPCRs. New insights into how these receptors work have re-energized the study of these receptors. Specifically, we study biased receptor signaling and GPCR heteromerization and potential overlap of these concepts and their roles in (patho)-physiological responses. We frequently use the delta opioid receptor as model receptor for our studies. We use a combination of in vitro cell culture assays, with in vivo mouse behavioral paradigms and ex vivo assays to perform translational/pre-clinical research with the goal of proposing and developing new therapeutic drugs that may have better potency, efficacy and fewer side effects. Our main focus is on neurological disorders, with an emphasis on drug addiction and co-morbid mood and anxiety disorders as well as chronic pain conditions. Current projects involve investigations into the risk of the alcohol mixed energy drinks, the role of beta-arrestin dependent signaling in depression and anxiety disorders, the physiological function of opioid-dopamine interactions and alcohol withdrawal induced hyperalgesia.

The Van Rijn lab: [Top from left to right] Arbaaz Mukadam, Ben Cummins, Rob Cassell, Maria Shelley, Richard van Rijn, Kendall Mores. [Bottom] Grace Mulia, Angel Lin, Haoyue Cui, Arryn Blaine, Anna Gutridge, Mee Jung Ko

Purdue Animal Care and Use committee member: 2015-present

MCMP Graduate admissions committee: 2015-present

Purdue University Life Sciences executive committee: 2017-present

Head of the Integrative Neuroscience training group: 2017-present  

College of Pharmacy Professional Program Admissions Committee: 2018-present

PHRM825: Integrated Pharmacotherapy I

PHRM844: Integrated Pharmacotherapy II

PHRM845: Integrated Pharmacotherapy III

PHRM316: Drug abuse/addiction education

National Insitute on Alcohol Abuse and Alcoholism (R21AA026675)  9/1/2018 - 08/31/2020

The influence of alcohol on disseminated tumor cells dormancy

The goal of this proposal is to investigate how alcohol use can influence systemic tumor dormancy following breast cancer metastasis

Role: Co-PI (With Dr. Wendt)

National Insitute on Alcohol Abuse and Alcoholism (R21AA026949)  8/1/2018 - 07/31/2020

Opioidergic alkaloids from Mitragynia Speciosa (kratom) as novel treatment for alcohol use disorder

The goal of this proposal is to study the impact of kratom and kratom alkaloids on alcohol use and reward in mice

Role: PI

National Institute on Drug Abuse (R03DA045897) 04/01/2018-03/31/2020

Development of G-protein biased delta opioid receptor agonists for inflammatory pain

The goal of this proposal is to synthesize novel G-protein biased delta opioid receptors to treat acute inflammatory pain

Role: PI

National Insitute on Alcohol Abuse and Alcoholism (R01AA025368)  10/1/2017 - 09/31/2022

G-protein-, beta-arrestin- and ERK-signaling in alcohol use- and anxiety-disorders

The primary objective of this grant is to to study the role of ERK signaling induced by G-protein or beta-arrestin pathways in modulation of alcohol and anxiety behaviors.

Role: PI

1. Ko MJ, Chiang T, Mukadam AM, Mulia, GE, Gutridge AM, Chester JA, Van Rijn RM, β-arrestin-dependent ERK signaling positively correlates with reduced anxiety-like and conditioned fear-related behavior in mice,  Science Signaling, 2021, TBD, accepted, https://doi.org/10.1101/790568
2. Creed SM¹, Gutridge AM¹, Argade MD, Hennessy MR, Brent Friesen JJB, Pauli GF, van Rijn RM^#, Riley AP^#, Isolation and Pharmacological Characterization of six Opioidergic Picralima nitida Alkaloids, J Natural products, 2021, TBD, accepted, https://doi.org/10.26434/chemrxiv.13007804.v1
3. Bertels Z, Witkowski W, Asif S, Siegersma K, Van Rijn RM, Pradhan AA, A non-convulsant delta-opioid receptor agonist, KNT-127, reduces cortical spreading depression and nitroglycerin-induced allodynia, Headache, 2021 TBD, accepted
4. Ding Z, Knipp GT, Van Rijn RM, Chester JA, Watts VJ, The CUL3/neddylation inhibitor MLN4924 reduces inflammatory pain allodynia and ethanol-induced locomotor sensitization in mice, Behavioural Brain Research, 2021, 399, 113051
5. ¹Gutridge AM, ¹Robins MT, ¹Cassel RJ, ¹Uprety R, Mores KL, Ko MJ, Pasternak GM, Majumdar S, Van Rijn RM, G protein-biased kratom-alkaloids and synthetic carfentanil-amide opioids as potential treatment for alcohol use disorder, Br J Pharmacol, 2020, 177(7): 1497-1513
6. Cai B, Kim D, Akhand S, Sun Y, Cassell RJ, Alpsoy A, Dykhuizen EC, Van Rijn RM, Wendt MK, Krusemark CJ, Selection of DNA-encoded libraries to protein targets within and on living cells, J Am Chem Soc, 2019, 141:17057-17061
7. ¹Mores KL, ¹Cummins BR, Cassell RJ, Van Rijn RM, A review of the therapeutic potential of recently developed G protein-biased kappa agonists, Front Pharmacol, 2019, 10 (407):1-14
8. ¹Ko MJ, ¹Mulia G, Van Rijn RM. Commonly used anesthesia/euthanasia methods for brain collection differentially impact MAPK activity in male and female C57BL/6 mice, Front Cell Neurosci, 2019, 13 (96):1-10
9. Robins MT, Blaine AT, Ha JE, Brewster A, Van Rijn RM, Repeated Use of the Psychoactive Substance Ethylphenidate Impacts Neurochemistry and Reward Learning in Adolescent Male and Female Mice. Front Neurosci,  2019, 13 (124): 1-14
10. ¹Ko MJ, ¹Ganzen L, Coskun E, Mukadam AA, Lueng YF, Van Rijn RM, Critical evaluation of TRPA1-mediated locomotive behavior in zebrafish as a screening tool for novel anti-nociceptive drug discovery. Sci Rep, 2019, 9 (2430): 1-11
11. ¹Cassell RJ, ¹Mores KL, Zerfas BL, AHA Abdallah, Lill MA, Trader DJ, Van Rijn RM, Rubsicolins are naturally occurring G-protein-biased delta opioid receptor peptide, Eur Neuropsychopharmacol, 2019, 29(3): 450-456 
12. Mores KL, Cassell RJ, van Rijn RM, arrestin recruitment and biased signaling by G protein-coupled receptor heteromers. Neuropharmacology (Special issue on Brain Heteroreceptor complexes in Health and Disease), accepted for publication,  https://doi.org/10.1016/j.neuropharm.2018.11.010
13. Kaur J, Soto-Velasquez M, Ding Z, Van Rijn RM, Watts VJ, Flaherty DP, Optimization of a 1,3,4-oxadiazole series for inhibition of adenylyl cyclase 1 and 8 for the treatment of chronic pain, 2019, Eur J Med Chem, 162, 565-585
14. Robins MT, Chiang T, Mores, KL, Alongkronrusmee D, Van Rijn RM, Critical role for G_i-protein activity in the dorsal striatum in the reduction of voluntary alcohol intake in C57Bl/6 mice, 2018, Front. Psychiatry (Special topics in opioid research), 9 (112): 1-14.
15. Robins MT, Chiang T, Berry JN, Ko MJ, Ha JE, Van Rijn RM, Behavioral characterization of b-arrestin 1 knockout mice in anxiety-like and alcohol behaviors, 2018, Front. Behav. Neurosci., 12 (54): 1-11
16. Márquez-Gómez R, Robins MT, Gutiérrez-Rodelo C, Arias, JM, Olivares-Reyes JA, Van Rijn RM, Arias-Montaño JA, Functional histamine H₃ and adenosine A_2A receptor heteromers in recombinant cells and rat striatum, 2018, Pharmacol. Res., 129: 515-525, PMID: 29217157
17. Brust TF, Alongkronrusmee D, Soto-Velasquez M, Baldwin TA, Ye Z, Dai, M, Dessauer CW, Van Rijn RM, Watts VJ, Identification of a selective small molecule inhibitor of type 1 adenylyl cyclase activity with analgesic properties, 2017, Science Signal, 10 (467): eaah5381, PMID:28223412. http://www.sciencemag.org/news/2017/02/new-class-painkillers-horizon
18. Zeng L, Alongkronrusmee D, Van Rijn RM, An integrative perspective on diabetic, alcoholic and drug-induced neuropathic pain, 2017, J Pain Res, 10: 219-228 PMID:28176937, PMC5268333. Noteworthy designation, highly accessed designation,https://www.dovepress.com/press_releases.php?content_id=3499
19. Alongkronrusmee D, Chiang T, Van Rijn RM, Involvement of delta opioid receptors in alcohol withdrawal-induced mechanical allodynia in male C57BL/6 mice. 2016, Drug Alcohol Depend, 167:190-198 PMID: 27567436
20. Yang S, Ben-Shalom R, Ahn M, Liptak AT, van Rijn RM, Whistler JL, Bender KJ, Beta-Arrestin-dependent dopaminergic regulation of calcium channel activity in the axon initial segment, 2016, Cell Rep, 16 (6), 1518-1526 PMID: 27452469, PMC5074334
21. Robins MT, Lu J, Van Rijn RM, Unique behavioral and neurochemical effects induced by repeated adolescent consumption of caffeine-mixed alcohol in C57BL/6 mice, 2016, PLoS One, 11(7):e0158189 PMID: 27380261, PMC4933367, Top 1% of most downloaded articles of the year (out of 22,000)
22. Robins MT, DeFriel JN, Van Rijn RM, adolescent intake of caffeinated energy drinks does not affect adult alcohol consumption in C57BL/6 and BALB/c mice, 2016, Alcohol, 54, 1-9, PMID:27565749
23. Chiang T, Sansuk K, Van Rijn RM, β-arrestin 2 dependence of delta opioid receptor agonists is correlated with alcohol intake., Br. J Pharmacol, 2016, 173, 332-343, PMID:26507558, PMC4940619
24. Van Rijn RM^#, DeFriel JN, Whistler JL, Pharmacological traits of delta opioid receptors: pitfalls or opportunities, Psychopharmacol, 2013, 228(1), 1-18, ^#corresponding author, PMID:23649885, PMC3679311
25. Milan-Lobo L, Enquist JE, Van Rijn RM, Whistler JL, anti-analgesic effect of the mu/delta opioid receptor-heteromer revealed by ligand-biased antagonism, 2013, PLoS One, 8(3): e58362, PMID:23554887, PMC3598907
26. Van Rijn RM^#, Harvey JH, Brissett DI, DeFriel JN, Whistler JL, Novel screening assay for the selective detection of G protein-coupled receptor heteromer signaling, 2013, J Pharmacol Exp Ther, 334(1):179-188, ^#corresponding author, PMID:23097213, PMC3533407, Highlighted paper
27. Van Rijn RM^#, Brissett DI, Whistler JL, Distinctive modulation of ethanol place preference by delta opioid receptor selective agonists, Drug Alcohol Depend, 2012, 122(1-2):156-159  ^#corresponding author, PMID:22018601, PMC3279630
28. Brissett DI, Whistler JL, Van Rijn RM^#, Contribution of mu and delta opioid receptors to the pharmacological profile of kappa opioid receptor subtypes, Eur. J Pain, 2012, 16 (3): 327-337. ^#corresponding author, PMID:22337177, PMC4086711
29. Van Rijn RM, Brissett DI, Whistler JL, Emergence of functional spinal delta-mu opioid receptors heteromers after chronic ethanol exposure, Biol Psychiatry, 2012, 71:232-238, PMID:21889123, PMC4086708
30. Van Rijn RM, Brissett DI, Whistler JL, Dual efficacy of delta opioid selective ligands for ethanol drinking and anxiety, J Pharmacol Exp Ther, 2010, 335 (1):133-139, PMID:20605909, PMC2957775
31. Van Rijn RM, Rhee IK, Verpoorte R, Isolation of acetylcholinesterase inhibitory alkaloids from Nerine Bowdenii, Nat. Prod. Res, 2010, 24 (3):222-225, PMID:20140801
32. Van Rijn RM, Whistler JL, Waldhoer M, Opioid receptor heteromer-specific trafficking and pharmacology, Curr Opin Pharmacol, 2010, 10:73-79, PMID:19846340, PMC2900797
33. Van Rijn RM, Whistler JL. The delta-1 opioid receptor is a heterodimer that opposes the actions of the delta-2 receptor on alcohol intake, Biol. Psychiatry, 2009, 66:777-784, PMID:19576572, PMC2757485
34. He L, Kim J, Ou C, McFadden W, van Rijn RM, Whistler JL, Methadone antinociception is dependent on peripheral opioid receptors, J Pain, 2009, 10 (4):369-379, PMID:19327642, PMC2952445
35. Van Rijn RM, Whistler JL, The only way is up: preventing opioid tolerance by promoting cell surface expression of MOR-DOR heterodimers? Mol. Interv, 2008, 8 (6): 277-280, PMID:19144899
36. Van Rijn RM, van Marle A, Chazot PL, Langemijer E, Qin Y, Shenton FC, Lim HD, Zuiderveld OP, Sansuk K, Dy M, Smit MJ, Tensen CP, Bakker RA, Leurs R, Cloning and characterization of dominant negative splice variants of the human histamine H₄ receptor, Biochem J, 2008, 414 (1):121-131, PMID:18452403
37. Van Rijn RM, Chazot PL, Shenton FC, Sansuk K, Bakker RA, Leurs R, Oligomerization of recombinant and endogenously expressed human histamine H₄ receptors, Mol. Pharmacol. 2006, 70(2):604-15, PMID: 16645125
38. Bakker RA, Lozada AF, van Marle A, Shenton FC, Drutel G, Karlstedt K, Hoffmann M, Lintunen M, Yamamoto Y, van Rijn RM, Chazot PL, Panula P, Leurs R, Discovery of naturally occurring splice variants of the rat histamine H₃ receptor that act as dominant negatives, Mol. Pharmacol, 2006, 69(4):1194-206, PMID:16415177
39. Lim, HD¹ and Van Rijn RM¹, Ling P, Bakker RA, Thurmond RL, Leurs R., Evaluation of histamine H₁-, H₂-, and H₃-receptor ligands at the human histamine H₄ receptor: identification of 4-methylhistamine as the first potent and selective H₄ receptor agonist. J Pharmacol Exp Ther. 2005, 4(3):1310-21. ¹shared first author, PMID:15947036 >300 citations
40. Terzioglu N, van Rijn RM, Bakker RA, De Esch IJ, Leurs R., Synthesis and structure-activity relationships of indole and benzimidazole piperazines as histamine H₄ receptor antagonists, Bioorg Med Chem Lett. 2004, 14(21):5251-6, PMID:15454206 >100 citations
41. Rhee IK, van Rijn RM, Verpoorte R., Qualitative determination of false-positive effects in the acetylcholinesterase assay using thin layer chromatography, Phytochem Anal, 2003, 14(3):127-31, PMID: 12793457 >100 citation