Proteomics Approaches to Explore the Proteome Structure, Function and Interactions
Proteomics has emerged as a powerful technique for molecular and cell biology research. It gives a picture of an organism's physiology at a given time, and can tell us how organisms respond to their environment or disease states. For over 10 years, Purdue Proteomics Facility (PPF) has provided innovative state-of-the-art mass spectrometry (MS) resources to support clinical, environmental, agriculture and plant biology research to gain key insights into the composition, regulation and function of molecular components and cellular pathways. As a shared facility of the Bindley Bioscience Center (BBC), the facility enables both targeted and global analysis of proteins and protein complexes, and for mapping their modifications and interactions. In this seminar, I will discuss different proteomics technologies available at the PPF for system-wide characterization of the cellular proteome. I will also discuss some of my recent proteomics works for mapping protein complex assembly and interactions in cells using different chromatographic separation techniques with quantitative MS profiling. I will also highlight some interesting results on phosphorylation dependent protein oligomerization.