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Department of Medicinal Chemistry and Molecular Pharmacology Personnel - Laurie Louise Parker

Laurie Louise Parker, Ph. D.
Adjunct Professor of Medicinal Chemistry and Molecular Pharmacology
E-mail: llparker@purdue.edu

Full directory listing
Picture of Laurie Louise Parker

Education

Postdoctoral Fellow, 2003-2008, University of Chicago (Stephen Kent/Stephen Kron)
Ph.D. Synthetic Organic Chemistry, 2003, University of Glasgow, Scotland (Prof. David Robins)
B.A. Chemistry, 2000, University of St. Thomas, St. Paul, MN

Research

Parker Lab Wiki Website

My research interests center on using chemical biology and proteomics to develop biosensors for intracellular phosphorylation signaling. We will use synthetic peptide and protein chemistry to design and synthesize probes for multiplexed analysis of cancer-related signaling proteins e.g. Abl, other Src-family kinases, receptor tyrosine kinases, etc. We will explore a wide variety of multidisciplinary analytical methods including mass spectrometry, fluorescence microscopy and flow cytometry to develop novel techniques for monitoring these signaling proteins and their activities. Our technology development goals will lead us to new methods for asking increasingly complex questions about multiple signaling activities, their disruption in cancer, and response of signaling profiles to cancer treatment.

Lab Members:

Frank Joy Ankudey (Graduate Student)
Wei Cui (Graduate Student)
Alyssa Jeanne Cramer Garrelts (Post-Doctoral Research Associate)
Steven Bradley Ouellette (PULSE Graduate Student)
Minervo Perez (Graduate Student)
Robert John Schuster (Graduate Student)
Tzu-Yi Yang (PULSE Graduate Student)

Teaching

Co-instructor for MCMP 204 Organic Chemistry I (with Prof. Marc Loudon).

Grants

2013 ABSCIEX Young Investigator Award (with Mark Hall)

2011-2013: R21CA160129 NIH/NCI Application of Emerging Technologies to Cancer Research

2011-2014: R21CA157395 NIH/NCI Innovative Technology Development for Cancer Research (Parker, PI/Irudayaraj, Co-PI)

2011-2012: ICTSI Core Pilot Grant

2010-2013: R00CA127161 NIH/NCI Pathway to Independence Award (R00 Phase)

2009-2011: ICTSI Project Development Team funding support

2009-2010: DOD/CDMRP Breast Cancer Concept Award (Parker, PI/Irudayaraj, Co-PI)

2009-2010: Showalter Trust Grant (Parker, PI/Rochet, Co-PI)

Representative Publications

Comprehensive PubMed listings

Damayanti, NP, Parker, LL*, Irudayaraj, JM* (*co-corresponding author) "Fluorescence lifetime imaging of biosensor peptide phosphorylation in single live cells," 52(14):3931-4 (2013)

Yang, TY, Eissler, CL, Hall, MC, Parker, LL. "A multiple reaction monitoring (MRM) method to detect Bcr-Abl kinase activity in CML using a peptide biosensor," PLoS One. 8(2):e56627 (2013)

Lipchik AM, Parker LL, "Time-resolved luminescence detection of spleen tyrosine kinase activity through terbium sensitization," Analytical Chemistry, 85(5):2582-8 (2013)

Lipchik, AM, Killins, RL, Geahlen, RL, Parker, LL, "A peptide-based biosensor assay to detect intracellular Syk kinase activation and inhibition" Biochemistry, 51(38):7515-24 (2012)

Tang J, Wang JY, Parker LL, “Detection of early Abl kinase activation after ionizing radiation by using a peptide biosensor” ChemBioChem, 13(5):665-73 (2012)

Martin VA, Wang WH, Lipchik AM, Parker LL, He Y, Zhang S, Zhang ZY, Geahlen RL, “Akt2 inhibits the activation of NFAT in lymphocytes by modulating calcium release from intracellular stores” Cell Cycle, 24(5):1064-73 (2012)

Bremmer SC, Hall H, Martinez JS, Eissler CL, Hinrichsen TH, Rossie S, Parker LL, Hall MC, Charbonneau H, “Cdc14 phosphatases preferentially dephosphorylate a subset of cyclin-dependent kinase (Cdk) sites containing phosphoserine” J. Biol. Chem., 287(3):1662-9 (2012)

Eissler, CL, Bremmer, SC, Martinez, JS, Parker, LL, Charbonneau, H and Hall, MC, “A general strategy for studying multisite protein phosphorylation using label-free selected reaction monitoring mass spectrometry” Analytical Biochemistry, 418(2):267-75

Placzek EA, Plebanek MP, Lipchik AM, Kidd SR, Parker LL, “A peptide biosensor for detecting intracellular Abl kinase activity using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry” Analytical Biochemistry, 397:73-8 (2010)
 
Parker, L. L. and Kron, S. J. Kinase activation in circulating cells: Opportunities for diagnosis and therapeutic monitoring, Expert Opinion on Medical Diagnostics, 2, 33 (2008).
 
Parker, L. L., Engel-Hall, A., Drew, K., Steinhardt, G., Helseth, D. L., Jabon, D., McMurry, T., Angulo, D. S., and Kron, S. J. Investigating quantitation of phosphorylation using MALDI-TOF mass spectrometry, Journal of Mass Spectrometry, 43, 518 (2008).
 
Parker, L. L., Kurutz, J., Kent, S. B. H., Kron, S. J. Control of the yeast cell cycle with a photocleavable alpha-factor analog, Angewandte Chemie International Edition, 45, 6322-25 (2006). Featured in Nature, Research Highlights, 443, 374 (2006) and ACS Chemical Biology, Spotlight, 9, 545 (2006)
 
Parker, L. L., Brueggemeier, S. B., Rhee, W. J., Wu, D., Kent, S. B. H., Kron, S. J. and Palecek S. P. Photocleavable peptide hydrogel arrays for MALDI-TOF analysis of kinase activity, The Analyst, 151, 1097-1104 (2006)
 
Parker, L. L., Kron, S. J., Kent, S. B. Optimizing thiophosphorylation in the presence of competing phosphorylation with MALDI-TOF-MS detection, Journal of Proteome Research, 4, 1863-66 (2005).
 
Parker, L. L., Lacy, S. M., Farrugia, L. J., Evans, C., Robins, D. J., O'Hare, C. C., Hartley, J. A., Jaffar, M., Stratford, I. J. A novel design strategy for stable metal complexes of nitrogen mustards as bioreductive prodrugs. Journal of Medicinal Chemistry, 47, 5683-89 (2004).

Curriculum Vitae

Click here for a full CV for Laurie Louise Parker. (an Adobe Acrobat file)

This record was last updated on Aug 12, 2014 at 4:14 PM
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