Laurie Louise Parker, Ph. D. Assistant Professor Phone: (765) 496-3974 E-mail: llparker@purdue.edu Full directory listing

Education

Research

My research interests center on using chemical biology and proteomics to develop biosensors for intracellular phosphorylation signaling. We will use synthetic peptide and protein chemistry to design and synthesize probes for multiplexed analysis of cancer-related signaling proteins e.g. Abl, other Src-family kinases, receptor tyrosine kinases, etc. We will explore a wide variety of multidisciplinary analytical methods including mass spectrometry, fluorescence microscopy and flow cytometry to develop novel techniques for monitoring these signaling proteins and their activities. Our technology development goals will lead us to new methods for asking increasingly complex questions about multiple signaling activities, their disruption in cancer, and response of signaling profiles to cancer treatment.

Teaching

Co-instructor for MCMP 204 Organic Chemistry I (with Prof. Marc Loudon).

Representative Publications

Placzek, E. A., Plebanek, M. P., Lipchik, A. M., Kidd S. R. and Parker, L. L. A peptide biosensor for detecting intracellular Abl kinase activity using MALDI-TOF MS, in press, Analytical Biochemistry (2009).

Parker, L. L. and Kron, S. J. Kinase activation in circulating cells: Opportunities for diagnosis and therapeutic monitoring, Expert Opinion on Medical Diagnostics, 2, 33 (2008).

Parker, L. L., Engel-Hall, A., Drew, K., Steinhardt, G., Helseth, D. L., Jabon, D., McMurry, T., Angulo, D. S., and Kron, S. J. Investigating quantitation of phosphorylation using MALDI-TOF mass spectrometry, Journal of Mass Spectrometry, 43, 518 (2008).

Parker, L. L., Kurutz, J., Kent, S. B. H., Kron, S. J. Control of the yeast cell cycle with a photocleavable alpha-factor analog, Angewandte Chemie International Edition, 45, 6322-25 (2006). Featured in Nature, Research Highlights, 443, 374 (2006) and ACS Chemical Biology, Spotlight, 9, 545 (2006)

Parker, L. L., Brueggemeier, S. B., Rhee, W. J., Wu, D., Kent, S. B. H., Kron, S. J. and Palecek S. P. Photocleavable peptide hydrogel arrays for MALDI-TOF analysis of kinase activity, The Analyst, 151, 1097-1104 (2006)

Parker, L. L., Kron, S. J., Kent, S. B. Optimizing thiophosphorylation in the presence of competing phosphorylation with MALDI-TOF-MS detection, Journal of Proteome Research, 4, 1863-66 (2005).

Parker, L. L., Lacy, S. M., Farrugia, L. J., Evans, C., Robins, D. J., O'Hare, C. C., Hartley, J. A., Jaffar, M., Stratford, I. J. A novel design strategy for stable metal complexes of nitrogen mustards as bioreductive prodrugs. Journal of Medicinal Chemistry, 47, 5683-89 (2004).