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Department of Medicinal Chemistry and Molecular Pharmacology Personnel - Tony R. Hazbun

Tony R. Hazbun, Ph.D.
Associate Professor of Medicinal Chemistry and Molecular Pharmacology
Phone: 765-496-8228
Fax: 765-494-1414

Full directory listing
Picture of Tony R. Hazbun
Specialization: Functional Genomics and Systems Biology, Chemical Genetics


B.S. 1989 - University of Queensland, Australia
Ph.D. 1997 - University of Notre Dame
Postdoc. 1998-2005 - University of Washington (Stanley Fields)

Research: Functional Genomics and Systems Biology, Chemical Genetics

Chromosomal instability due to aberrant chromosome segregation is a hallmark of cancer, suggesting that a more detailed and mechanistic understanding of the molecular processes that control the faithful segregation of chromosomes will lead to new therapeutic strategies to control cancer. An example of this promise is evident in the Aurora kinases, which are important regulators of chromosome segregation. Several specific Aurora kinase inhibitors have demonstrated anti-proliferative activities and are proceeding to clinical trials even though we do not understand the multiple roles of this kinase. Our research involves the development of functional genomics approaches in baker's yeast, Saccharomyces cerevisiae, to focus on the kinase-signaling network of the yeast Aurora kinase, Ipl1. The downstream effects these phosphorylated substrates have on protein-protein interactions will be determined using an integrated approach relying on a phosphomutant scanning approach in conjunction with two-hybrid technology and systematic genetic studies. Identification of phosphomodulated genetic interactions and phosphomodulated protein-protein interactions related to Ipl1 and it's control of mitosis and chromosome segregation in yeast, should serve as a guide toward the analysis of and ultimately, intervention in, the abnormal mitotic pathways that propagate cancer cells.

Lab Members:

Kiran Aslam (Graduate Student)
Kourtney Michelle Goode (Graduate Student)
Kriti Shrestha (Graduate Student)


  • Mitosis and Cancer
  • Chromosome segregation
  • Aurora kinases
  • Hsp90


  • MCMP 422 - Immunology
  • PHRM 821 Professional Program Laboratory II
  • MCMP 514 - Advanced Medicinal Analysis
  • MCMP 690G - Molecular Targets: Cancer

Honors and Credentials

Positions and Employment

1998-2005       Howard Hughes Medical Institute, Postdoctoral Associate, University of Washington

2001-2005       Two-hybrid Screening Coordinator, Yeast Resource Center (NCRR), University of Washington

2005-2011       Assistant Professor of Medicinal Chemistry and Molecular Pharmacology, Purdue University

2005-present  Full Member of the Bindley Bioscience Center, Discovery Park, Purdue University

2011-present  Associate Professor of Medicinal Chemistry and Molecular Pharmacology, Purdue University


Other Experience and Professional Memberships

1994-1997       Teaching Assistant, Department of Biology, University of Notre Dame 

2003                Future Faculty Fellows program, HHMI sponsored workshop

2005-present  Instructor – Immunology for Pharm.D. students

2005-present  Instructor – Cell Cycle and Drug Discovery for Graduate Students

Professional memberships Genetics Society of America, ABMB


Ad hoc reviewing activities

Reviewed for Nature Biotechnology, Biochemistry, PNAS, PLoS Genetics, PLoS Pathogens, PLoS One, Protein Science, Genome Biology, Cold Spring Harbor Laboratories Protocol, Biotechniques, Bioinformatics, Assay Development and Technologies, Genes Genomes and Genetics (G3), Methods, Wiley Encyclopedia of Chemical Biology.

Reviewed grants for:
National Institute of Health (NIH) – Genomics, Computational Biology and Technology Study Section

National Science Foundation (NSF)

Biomedical Research Council A*STAR – Singapore

Civilian Research and Development Foundation (CRDF)

New Zealand Ministry of Science and Innovation (MSI)


"Integrated Genomewide Analysis of the Ipl1 Kinase Signaling Network", 7/01/2009-6/30/2014, R01 funded by NIH, NIGMS

"High-throughput Chemotyping of Yeast Signature Strains Reflecting Hsp90 Biology", 09/30/2007 - 05/31/2010, R21 MLPCN funded by NIH, NINDS; 1R21NS061667-01 (co-investigator: V Jo Davisson, Purdue University)

PhRMA Foundation: Uncovering the Proteomic Network Surrounding the Yeast Aurora Kinase, Ipl

Administration and Committee Work

PULSe Molecular Signaling and Cancer Biology Training Group Chair

MCMP Graduate Admissions Committee Chair



Sept 2012        Molecular, Cellular and Developmental Biology Series, Indiana University, Bloomington, IN

Oct  2011        Midwest Yeast Meeting, Northwestern University

Apr  2011       School of Pharmaceutical Sciences, University of Wisconsin, Madison, Wisconsin

Dec  2010        Human Proteome Organisation (HUPO) – Invited educational tutorial and publication

Nov 2010        Department of Biological Sciences, Valparaiso University, Indiana

July 2010        Genetics Society of America Yeast Genetics and Molecular Biology Conference, Vancouver, BC

July 2010        Mid-Atlantic Graduate Student Symposium in Medicinal Chemistry, Purdue University

Mar 2010        Biochemistry Department, Michigan State University, East Lansing, MI

Nov 2008        Science Education in Computational Thinking (SECANT), Purdue University

Jul 2007          XXIII International Conference on Yeast Genetics & Molecular Biology, Melbourne, Australia

Representative Publications

  • Tsai CJ, Aslam K, Drendel HM, Asiago JM, Goode KM, et al. Hsp31 is a Stress-Response Chaperone that Intervenes in the Protein Misfolding Process. J Biol Chem. 2015 Aug 25;PubMed PMID: 26306045.
  • Thapa KS, Oldani A, Pagliuca C, De Wulf P, Hazbun TR. The Mps1 kinase modulates the recruitment and activity of Cnn1(CENP-T) at Saccharomyces cerevisiae kinetochores. Genetics. 2015 May;200(1):79-90. PubMed PMID: 25716979; PubMed Central PMCID: PMC4423383.
  • Mukherjee D, Sen A, Boettner DR, Fairn GD, Schlam D, et al. Bem3, a Cdc42 GTPase-activating protein, traffics to an intracellular compartment and recruits the secretory Rab GTPase Sec4 to endomembranes. J Cell Sci. 2013 Oct 15;126(Pt 20):4560-71. PubMed PMID: 23943876; PubMed Central PMCID: PMC3795334.
  • Song B, Liu XS, Rice SJ, Kuang S, Elzey BD, et al. Plk1 phosphorylation of orc2 and hbo1 contributes to gemcitabine resistance in pancreatic cancer. Mol Cancer Ther. 2013 Jan;12(1):58-68. PubMed PMID: 23188630; NIHMSID: NIHMS424712; PubMed Central PMCID: PMC3732037.
  • Bock LJ, Pagliuca C, Kobayashi N, Grove RA, Oku Y, et al. Cnn1 inhibits the interactions between the KMN complexes of the yeast kinetochore. Nat Cell Biol. 2012 May 6;14(6):614-24. PubMed PMID: 22561345; NIHMSID: UKMS49793; PubMed Central PMCID: PMC3438452.
  • Ren L, McLean JR, Hazbun TR, Fields S, Vander Kooi C, et al. Systematic two-hybrid and comparative proteomic analyses reveal novel yeast pre-mRNA splicing factors connected to Prp19. PLoS One. 2011 Feb 28;6(2):e16719. PubMed PMID: 21386897; PubMed Central PMCID: PMC3046128.
  • Luo J, Xu X, Hall H, Hyland EM, Boeke JD, et al. Histone h3 exerts a key function in mitotic checkpoint control. Mol Cell Biol. 2010 Jan;30(2):537-49. PubMed PMID: 19917722; PubMed Central PMCID: PMC2798460.
  • Wong J, Nakajima Y, Westermann S, Shang C, Kang JS, et al. A protein interaction map of the mitotic spindle. Mol Biol Cell. 2007 Oct;18(10):3800-9. PubMed PMID: 17634282; PubMed Central PMCID: PMC1995735.
  • Jin F, Avramova L, Huang J, Hazbun T. A yeast two-hybrid smart-pool-array system for protein-interaction mapping. Nat Methods. 2007 May;4(5):405-7. PubMed PMID: 17450148; NIHMSID: NIHMS23621; PubMed Central PMCID: PMC2771206.
  • Hazbun TR, Miller JP. Genome-Wide Analysis of Protein-Protein Interactions Using a Two-Hybrid Array: Amplification of ORFs. CSH Protoc. 2006 Sep 1;2006(4)PubMed PMID: 22485925.
  • Hazbun TR, Miller JP. Genome-wide analysis of protein-protein interactions using a two-hybrid array: cloning by homologous recombination and high-throughput transformation. CSH Protoc. 2006 Sep 1;2006(4)PubMed PMID: 22485926.
  • Hazbun TR, Miller JP. Genome-wide analysis of protein-protein interactions using a two-hybrid array: high-density robotic screening procedure. CSH Protoc. 2006 Sep 1;2006(4)PubMed PMID: 22485927.
  • Hazbun TR, Miller JP. Genome-wide analysis of protein-protein interactions using a two-hybrid array: storage, maintenance, and working with living arrays. CSH Protoc. 2006 Sep 1;2006(4)PubMed PMID: 22485928.
  • Montpetit B, Hazbun TR, Fields S, Hieter P. Sumoylation of the budding yeast kinetochore protein Ndc10 is required for Ndc10 spindle localization and regulation of anaphase spindle elongation. J Cell Biol. 2006 Aug 28;174(5):653-63. PubMed PMID: 16923829; PubMed Central PMCID: PMC2064309.
  • Jin F, Hazbun T, Michaud GA, Salcius M, Predki PF, et al. A pooling-deconvolution strategy for biological network elucidation. Nat Methods. 2006 Mar;3(3):183-9. PubMed PMID: 16489335; NIHMSID: NIHMS165288; PubMed Central PMCID: PMC2803036.

This record was last updated on Oct 6, 2015 at 10:36 AM
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