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Department of Medicinal Chemistry and Molecular Pharmacology Personnel - Tony R. Hazbun

Tony R. Hazbun, Ph.D.
Associate Professor of Medicinal Chemistry and Molecular Pharmacology
Phone: 765-496-8228
Fax: 765-494-1414
E-mail: thazbun@purdue.edu

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Picture of Tony R. Hazbun
Specialization: Functional Genomics and Systems Biology, Chemical Genetics

Education

B.S. 1989 - University of Queensland, Australia
Ph.D. 1997 - University of Notre Dame
Postdoc. 1998-2005 - University of Washington (Stanley Fields)

Research: Functional Genomics and Systems Biology, Chemical Genetics

Chromosomal instability due to aberrant chromosome segregation is a hallmark of cancer, suggesting that a more detailed and mechanistic understanding of the molecular processes that control the faithful segregation of chromosomes will lead to new therapeutic strategies to control cancer. An example of this promise is evident in the Aurora kinases, which are important regulators of chromosome segregation. Several specific Aurora kinase inhibitors have demonstrated anti-proliferative activities and are proceeding to clinical trials even though we do not understand the multiple roles of this kinase. Our research involves the development of functional genomics approaches in baker's yeast, Saccharomyces cerevisiae, to focus on the kinase-signaling network of the yeast Aurora kinase, Ipl1. The downstream effects these phosphorylated substrates have on protein-protein interactions will be determined using an integrated approach relying on a phosphomutant scanning approach in conjunction with two-hybrid technology and systematic genetic studies. Identification of phosphomodulated genetic interactions and phosphomodulated protein-protein interactions related to Ipl1 and it's control of mitosis and chromosome segregation in yeast, should serve as a guide toward the analysis of and ultimately, intervention in, the abnormal mitotic pathways that propagate cancer cells.

Lab Members:

Kiran Aslam (Graduate Student)
Kourtney Michelle Fultz (Graduate Student)
Kriti Shrestha (Graduate Student)

Interests

  • Mitosis and Cancer
  • Chromosome segregation
  • Aurora kinases
  • Hsp90

Teaching

  • MCMP 422 - Immunology
  • PHRM 821 Professional Program Laboratory II
  • MCMP 514 - Advanced Medicinal Analysis
  • MCMP 690G - Molecular Targets: Cancer

Honors and Credentials

Positions and Employment

1998-2005       Howard Hughes Medical Institute, Postdoctoral Associate, University of Washington

2001-2005       Two-hybrid Screening Coordinator, Yeast Resource Center (NCRR), University of Washington

2005-2011       Assistant Professor of Medicinal Chemistry and Molecular Pharmacology, Purdue University

2005-present  Full Member of the Bindley Bioscience Center, Discovery Park, Purdue University

2011-present  Associate Professor of Medicinal Chemistry and Molecular Pharmacology, Purdue University

 

Other Experience and Professional Memberships

1994-1997       Teaching Assistant, Department of Biology, University of Notre Dame 

2003                Future Faculty Fellows program, HHMI sponsored workshop

2005-present  Instructor – Immunology for Pharm.D. students

2005-present  Instructor – Cell Cycle and Drug Discovery for Graduate Students

Professional memberships Genetics Society of America, ABMB

 

Ad hoc reviewing activities

Reviewed for Nature Biotechnology, Biochemistry, PNAS, PLoS Genetics, PLoS Pathogens, PLoS One, Protein Science, Genome Biology, Cold Spring Harbor Laboratories Protocol, Biotechniques, Bioinformatics, Assay Development and Technologies, Genes Genomes and Genetics (G3), Methods, Wiley Encyclopedia of Chemical Biology.

Reviewed grants for:
National Institute of Health (NIH) – Genomics, Computational Biology and Technology Study Section

National Science Foundation (NSF)

Biomedical Research Council A*STAR – Singapore

Civilian Research and Development Foundation (CRDF)

New Zealand Ministry of Science and Innovation (MSI)

Grants


"Integrated Genomewide Analysis of the Ipl1 Kinase Signaling Network", 7/01/2009-6/30/2013, R01 funded by NIH, NIGMS

"High-throughput Chemotyping of Yeast Signature Strains Reflecting Hsp90 Biology", 09/30/2007 - 05/31/2010, R21 MLPCN funded by NIH, NINDS; 1R21NS061667-01 (co-investigator: V Jo Davisson, Purdue University)

PhRMA Foundation: Uncovering the Proteomic Network Surrounding the Yeast Aurora Kinase, Ipl

Showalter Trust: DJ-1: an "ON" for Cell Survival Pathways in the Brain, (co-investigator: J-C Rochet, Purdue University)

Showalter Trust: Identification of Novel Anti-cancer Drug Targets in Cells Overexpressing Lyar, (co-investigator: S Mendrysa, Purdue University)

Showalter Trust: Cell Cycle Regulatory Functions of the 14-3-3 Protein Family, (co-investigators: Mark Hall and Harry Charbonneau, Purdue University)

Administration and Committee Work

PULSe Molecular Signaling and Cancer Biology Training Group Chair

MCMP Graduate Admissions Committee Chair

Presentations

 

Sept 2012        Molecular, Cellular and Developmental Biology Series, Indiana University, Bloomington, IN

Oct  2011        Midwest Yeast Meeting, Northwestern University

Apr  2011       School of Pharmaceutical Sciences, University of Wisconsin, Madison, Wisconsin

Dec  2010        Human Proteome Organisation (HUPO) – Invited educational tutorial and publication

Nov 2010        Department of Biological Sciences, Valparaiso University, Indiana

July 2010        Genetics Society of America Yeast Genetics and Molecular Biology Conference, Vancouver, BC

July 2010        Mid-Atlantic Graduate Student Symposium in Medicinal Chemistry, Purdue University

Mar 2010        Biochemistry Department, Michigan State University, East Lansing, MI

Nov 2008        Science Education in Computational Thinking (SECANT), Purdue University

Jul 2007          XXIII International Conference on Yeast Genetics & Molecular Biology, Melbourne, Australia

Representative Publications

1. Song B, Liu XS, Rice SJ, Kuang S, Elzey BD, Konieczny SF, Ratliff TL, Hazbun T, Chiorean EG, & Liu X (2013) Plk1 phosphorylation of orc2 and hbo1 contributes to gemcitabine resistance in pancreatic cancer. Molecular cancer therapeutics 12(1):58-68.

2. Mukherjee D, Sen A, Boettner DR, Fairn GD, Schlam D, Bonilla Valentin FJ, McCaffery JM, Hazbun T, Staiger CJ, Grinstein S, Lemmon SK, & Aguilar RC (2013) Bem3, a Cdc42 GTPase-Activating Protein, traffics to an intracellular compartment and recruits the secretory Rab GTPase Sec4 to endomembranes. Journal of cell science.

3. Bock LJ, Pagliuca C, Kobayashi N, Grove RA, Oku Y, Shrestha K, Alfieri C, Golfieri C, Oldani A, Dal Maschio M, Bermejo R, Hazbun TR, Tanaka TU, & De Wulf P (2012) Cnn1 inhibits the interactions between the KMN complexes of the yeast kinetochore. Nature cell biology 14(6):614-624.

4. Ren L, McLean JR, Hazbun TR, Fields S, Vander Kooi C, Ohi MD, & Gould KL (2011) Systematic two-hybrid and comparative proteomic analyses reveal novel yeast pre-mRNA splicing factors connected to Prp19. PloS one 6(2):e16719.

5. Luo J, Xu X, Hall H, Hyland EM, Boeke JD, Hazbun T, & Kuo MH (2010) Histone h3 exerts a key function in mitotic checkpoint control. Molecular and cellular biology 30(2):537-549.

6. Wong J, Nakajima Y, Westermann S, Shang C, Kang JS, Goodner C, Houshmand P, Fields S, Chan CS, Drubin D, Barnes G, & Hazbun T (2007) A protein interaction map of the mitotic spindle. Molecular biology of the cell 18(10):3800-3809.

7. Jin F, Avramova L, Huang J, & Hazbun T (2007) A yeast two-hybrid smart-pool-array system for protein-interaction mapping. Nature methods 4(5):405-407.

8. Montpetit B, Hazbun TR, Fields S, & Hieter P (2006) Sumoylation of the budding yeast kinetochore protein Ndc10 is required for Ndc10 spindle localization and regulation of anaphase spindle elongation. The Journal of cell biology 174(5):653-663.

9. Jin F, Hazbun T, Michaud GA, Salcius M, Predki PF, Fields S, & Huang J (2006) A pooling-deconvolution strategy for biological network elucidation. Nature methods 3(3):183-189.

10. Hazbun TR & Miller JP (2006) Genome-Wide Analysis of Protein-Protein Interactions Using a Two-Hybrid Array: Amplification of ORFs. CSH protocols 2006(4).

11. Hazbun TR & Miller JP (2006) Genome-wide analysis of protein-protein interactions using a two-hybrid array: cloning by homologous recombination and high-throughput transformation. CSH protocols 2006(4).

12. Hazbun TR & Miller JP (2006) Genome-wide analysis of protein-protein interactions using a two-hybrid array: high-density robotic screening procedure. CSH protocols 2006(4).

13. Hazbun TR & Miller JP (2006) Genome-wide analysis of protein-protein interactions using a two-hybrid array: storage, maintenance, and working with living arrays. CSH protocols 2006(4).

14. Gingras AC, Caballero M, Zarske M, Sanchez A, Hazbun TR, Fields S, Sonenberg N, Hafen E, Raught B, & Aebersold R (2005) A novel, evolutionarily conserved protein phosphatase complex involved in cisplatin sensitivity. Molecular & cellular proteomics : MCP 4(11):1725-1740.

15. Guo D, Hazbun TR, Xu XJ, Ng SL, Fields S, & Kuo MH (2004) A tethered catalysis, two-hybrid system to identify protein-protein interactions requiring post-translational modifications. Nature biotechnology 22(7):888-892.

16. Shang C, Hazbun TR, Cheeseman IM, Aranda J, Fields S, Drubin DG, & Barnes G (2003) Kinetochore protein interactions and their regulation by the Aurora kinase Ipl1p. Molecular biology of the cell 14(8):3342-3355.

17. Hazbun TR, Malmstrom L, Anderson S, Graczyk BJ, Fox B, Riffle M, Sundin BA, Aranda JD, McDonald WH, Chiu CH, Snydsman BE, Bradley P, Muller EG, Fields S, Baker D, Yates JR, 3rd, & Davis TN (2003) Assigning function to yeast proteins by integration of technologies. Molecular cell 12(6):1353-1365.

18. Hazbun TR & Fields S (2002) A genome-wide screen for site-specific DNA-binding proteins. Molecular & cellular proteomics : MCP 1(7):538-543.

19. Gera JF, Hazbun TR, & Fields S (2002) Array-based methods for identifying protein-protein and protein-nucleic acid interactions. Methods in enzymology 350:499-512.

20. Hazbun TR & Fields S (2001) Networking proteins in yeast. Proceedings of the National Academy of Sciences of the United States of America 98(8):4277-4278.

21. Jana R, Hazbun TR, Fields JD, & Mossing MC (1998) Single-chain lambda Cro repressors confirm high intrinsic dimer-DNA affinity. Biochemistry 37(18):6446-6455.

22. Jana R, Hazbun TR, Mollah AK, & Mossing MC (1997) A folded monomeric intermediate in the formation of lambda Cro dimer-DNA complexes. Journal of molecular biology 273(2):402-416.

23. Hazbun TR, Stahura FL, & Mossing MC (1997) Site-specific recognition by an isolated DNA-binding domain of the sine oculis protein. Biochemistry 36(12):3680-3686.

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