Department of Medicinal Chemistry and Molecular Pharmacology Personnel - Donald E. Bergstrom
Specialization: Medicinal and Bioorganic Chemistry of Nucleic Acids
EducationB.S. - 1965 University of Washington
Ph.D. - 1970 University of California Berkeley
Postdoc. - 1970-72 University of Illinois
Research: Medicinal and Bioorganic Chemistry of Nucleic Acids
Our research is directed towards the construction of modified nucleic acids for use as biochemical tools, diagnostic probes, and therapeutics. This research requires the design and synthesis of nucleic acid components as well as novel molecular probes. For example, we are engaged in the development of a new generation of molecular end-caps designed to stabilize short double-stranded DNA segments (dsDNA). Capped dsDNA facilitates crystallization of protein-DNA complexes. In addition, endcaps are being investigated as structural elements for directing the folding and stabilizing G-quadruplexes and as components of therapeutic oligonucleotides, including decoy oligonucleotides directed towards STAT3, a transcription factor of significance to the etiology of head and neck cancer.
Carbon nanotubes have been shown to function as water-soluble carriers for biological molecules. These are taken up into cells and have been proposed as cell-targeting therapeutic-delivery platforms. In collaboration with Professors Ron Reifenberger (Physics) and David Riese (Medicinal Chemistry and Molecular Pharmacology), we have prepared DNA conjugated single wall carbon nanotubes and are studying the potential of these as siRNA carriers specific for EGF receptor, which is over-expressed in cancer cells.
Working in collaboration with Ernest R. Blatchley III (School of Civil Engineering) and J. Paul Robinson (School of Veterinary Medicine), we have developed a microsphere coated with the compound (E)-5-[2-(methoxy-carbonyl)ethenyl]cytidine for UV dosimetry. On UV irradiation the coated microspheres become fluorescent. This attribute allows their application as a Lagrangian actinometer for estimation of dose distributions and process performance in polychromatic UV disinfection system
Working with Professor Rashid Bashir (School of Electrical and Computer Engineering) we are developing nanowires for quantifying microRNAs and proteins overexpressed in cancer. Our segment of the project is focused on the development of methodology for the functionalization of silicon nanowires with peptide nucleic acids (PNA). In addition, we are building PNA derived nanoparticles as components of cancer therapeutic agents with Professor James Leary (Weldon School of Biomedical Engineering).
Bobby Reddy, Jr., Oguz H. Elibol, O Pradeep R. Nair,, Brian R. Dorvel, Felice Butler, X Zahab Ahsan, Donald E. Bergstrom, X Muhammad A. Alam, and Rashid Bashir, Silicon Field Effect Transistors as Dual-Use Sensor-Heater Hybrids, Anal. Chem., 2011, dx.doi.org/10.1021/ac102566f.
B. Dorvel, B. Reddy, Jr., I. Block, P. Mathias, S. E. Clare, B. Cunningham, D. E. Bergstrom, R. Bashir, " Vapor-Phase Deposition of Monofunctional Alkoxysilanes for Sub-Nanometer-Level Biointerfacing on Silicon Oxide Surfaces," Advanced Functional Materials, 2010, 20, 87-95.
T. Bhuvana1, W. Boley, B. Radha, B.D. Dolash, G. Chiu, D. Bergstrom, R. Reifenberger, T.S. Fisher, G.U. Kulkarni, "Inkjet printing of palladium alkanethiolates for facile fabrication of metal interconnects and surface-enhanced Raman scattering substrates," Micro & Nano Letters, 2010, 5, 296 – 299.
Brian M. Laing, Lisa Barrow-Laing, Maureen Harrington, Eric C. Long, and Donald E. Bergstrom, "Properties of Double-Stranded Oligonucleotides Modified with Lipophilic Substituents." Bioconjugate Chem. 2010, 21, 1537–1544.
Pei-Sze Ng, Brian M. Laing, Ganesan Balasundarum, Maneesh Pingle, Alan Friedman, and Donald E. Bergstrom, "Synthesis and Evaluation of New Spacers for Use as dsDNA End-Caps," Bioconjugate Chem. 2010, 21, 1545–1553.
Brian M. Laing, Peixuan Guo, and Donald E. Bergstrom, "Optimized Method for the Synthesis and Purification of Adenosine - Folic Acid Conjugates for use as Transcription Initiators in the Preparation of Modified RNA," Methods, 2010, doi:10.1016/j.ymeth.2010.12.007.
O. H. Elibol, B. Reddy Jr., P. R. Nair, B. Dorvel, F. Butler, Z. S. Ahsan, D. E. Bergstrom, M. A. Alam and Rashid Bashir, "Localized heating on silicon field effect transistors: Device fabrication and temperature measurements in fluid," Lab on a Chip, 2009, 9, 2789-2795, DOI: 10.1039/b906048k.
Roya R. Lahiji, Bridget D. Dolash, Jennifer McDonald, Dmitry Zemlyanov, Donald E. Bergstrom, and Ronald Reifenberger, "Electrodeposition study of ODN:SWCNT hybrids on gold substrates." Physica Status Solidi 2008, 205, 1408-1411.
M. Ahmadian and Donald E. Bergstrom, "5-Substituted Nucleosides in Biochemistry and Biotechnology." In Modified Nucleosides in Biochemistry, Biotechnoloy and Medicine, P. Herdewijn, ed. Wiley-VCH, Weihheim, 2008, pp 251-276.
Shiyue Fang, Yousheng Guan, Ernest R. Blatchley III, Chengyue Shen, and Donald E. Bergstrom, "Conjugation of (E)-5-[2-(Methoxycarbonyl)ethenyl]cytidine to Hydrophilic Microspheres: Development of a Mobile Microscale UV Light Actinometer", Bioconjugate Chemistry, 2008, 19, 592-597.
This record was last updated on May 18, 2012 at 9:07 AM