Department of Medicinal Chemistry and Molecular Pharmacology Personnel - Casey J. Krusemark
Specialization: Chemical biology, DNA-programmed combinatorial chemistry
EducationPostdoctoral Fellow, 2007-2013, Stanford University (Pehr Harbury/Patrick O. Brown)
Ph.D. Biochemistry, 2007, University of Wisconsin--Madison (Peter Belshaw)
B.S. Chemistry, B.S. Crop Science, 2001, University of Illinois--Urbana-Champaign
Research: Chemical biology, DNA-programmed combinatorial chemistry
Research in the Krusemark laboratory focuses on the discovery of functional small molecules using the power of in vitro evolution. DNA-programmed combinatorial chemistry (DPCC) enables the "translation" of DNA constructs into DNA conjugates with wholly synthetic small molecules, thus allowing Darwinian-like selections. DPCC allows the synthesis and functional interrogation of small molecule libraries of unprecedented size and complexity (presently up to 10^10 members).
The lab works of the further development of the DPCC technology towards the goal of a streamlined system for generating drug leads and biological tool compounds. We use a combination of chemical, engineering, and molecular biology methods. We are working to expand the synthetic toolbox available for builiding molecules on unprotected DNA to produce molecules with more drug-like properties. Our lab will develop tools to study the protein kinase C (PKC) family of kinases, which are involved in the signal transduction pathways of numerous and varied cellular processes.
Lab Members:Kyle Edward Denton (Graduate Student)
Rachael R. Jetson (Post-Doctoral Research Associate)
MCMP 204 Organic Chemistry I
Krusemark, C. J., Tilmans, N. P., Brown, P. O., Harbury, P. B., "Quantitative Population Behavior in Chemical Evolution." In review.
Tilmans, N. P., Krusemark, C. J., Harbury, P. B., “Expedient Synthesis of a Modular Phosphate Affinity Reagent.” Bioconj. Chem. 2010, 21, 1010-1013.
Frey, B. L., Ladror, D. T., Sondalle, S. B., Krusemark, C. J., Jue, A. L., Coon, J. J., Smith, L. M., “Chemical derivatization of peptide carboxyl groups for highly efficient electron transfer dissociation." J. Am. Soc. Mass Spectrom. 2013, 24, 1710-1721.
Krusemark, C. J., Frey, B. L., Belshaw, P. B., Smith, L. M., "Modifying the Charge State Distribution of Proteins in Electrospray Ionization Mass Spectrometry by Chemical Derivatization." J. Am. Soc. Mass Spectrom. 2009, 20, 1617-1625.
Krusemark, C. J., Ferguson, J. T., Wenger, C. D., Kelleher, N. L., Belshaw, P. J., "Global Amine and Acid Functional Group Modification of Proteins." Analytical Chemistry. 2008, 80, 713-720.
Krusemark, C. J. and Belshaw, P. J., "Covalent Labelling of Fusion Proteins in Live Cells via an Engineered Receptor-Ligand Pair." Organic and Biomolecular Chemistry. 2007, 5, 2201-2204.
Krusemark, C. J.*, Lamos, S. M.*, McGee, C. J., Scalf, M., Smith, L. M., Belshaw, P. J., "Mixed Isotope Photoaffinity Reagents for Identification of Small Molecule Targets by Mass Spectrometry." Angewandte Chemie Int. Ed. 2006, 45, 4329-4333. *Authors contributed equally.
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This record was last updated on Dec 21, 2013 at 1:28 PM