Department of Medicinal Chemistry and Molecular Pharmacology Personnel - Casey J Krusemark
Specialization: Chemical biology, DNA-programmed combinatorial chemistry
EducationPostdoctoral Fellow, 2007-2013, Stanford University (Pehr Harbury/Patrick O. Brown)
Ph.D. Biochemistry, 2007, University of Wisconsin--Madison (Peter Belshaw)
B.S. Chemistry, B.S. Crop Science, 2001, University of Illinois--Urbana-Champaign
Research: Chemical biology, DNA-programmed combinatorial chemistry
Research in the Krusemark laboratory focuses on the discovery of functional small molecules using the power of in vitro evolution. DNA-programmed combinatorial chemistry (DPCC) enables the "translation" of DNA constructs into DNA conjugates with wholly synthetic small molecules, thus allowing Darwinian-like selections. DPCC allows the synthesis and functional interrogation of small molecule libraries of unprecedented size and complexity (presently up to 10^10 members).
The lab works of the further development of the DPCC technology towards the goal of a streamlined system for generating drug leads and biological tool compounds. We use a combination of chemical, engineering, and molecular biology methods. We are working to expand the synthetic toolbox available for builiding molecules on unprotected DNA to produce molecules with more drug-like properties. Our lab will develop tools to study the protein kinase C (PKC) family of kinases, which are involved in the signal transduction pathways of numerous and varied cellular processes.
Krusemark, C. J., Tilmans, N. P., Brown, P. O., Harbury, P. B., "Quantitative Population Behavior in Chemical Evolution." Nucleic Acids Research. In review.
Tilmans, N. P., Krusemark, C. J., Harbury, P. B., “Expedient Synthesis of a Modular Phosphate Affinity Reagent.” Bioconj. Chem. 2010, 21, 1010-1013.
Frey, B. L., Ladror, D. T., Sondalle, S. B., Krusemark, C. J., Jue, A. L., Coon, J. J., Smith, L. M., “Chemical derivatization of peptide carboxyl groups for highly efficient electron transfer dissociation." J. Am. Soc. Mass Spectrom. In press.
Krusemark, C. J., Frey, B. L., Belshaw, P. B., Smith, L. M., "Modifying the Charge State Distribution of Proteins in Electrospray Ionization Mass Spectrometry by Chemical Derivatization." J. Am. Soc. Mass Spectrom. 2009, 20, 1617-1625.
Krusemark, C. J., Ferguson, J. T., Wenger, C. D., Kelleher, N. L., Belshaw, P. J., "Global Amine and Acid Functional Group Modification of Proteins." Analytical Chemistry. 2008, 80, 713-720.
Krusemark, C. J. and Belshaw, P. J., "Covalent Labelling of Fusion Proteins in Live Cells via an Engineered Receptor-Ligand Pair." Organic and Biomolecular Chemistry. 2007, 5, 2201-2204.
Krusemark, C. J.*, Lamos, S. M.*, McGee, C. J., Scalf, M., Smith, L. M., Belshaw, P. J., "Mixed Isotope Photoaffinity Reagents for Identification of Small Molecule Targets by Mass Spectrometry." Angewandte Chemie Int. Ed. 2006, 45, 4329-4333. *Authors contributed equally.
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This record was last updated on Aug 21, 2013 at 2:01 PM