Department of Medicinal Chemistry and Molecular Pharmacology Personnel - Casey J. Krusemark
Specialization: Chemical biology, DNA-encoded medicinal chemistry, Proteomics
EducationPostdoctoral Fellow, 2007-2013, Stanford University (under Pehr Harbury and Patrick O. Brown)
Ph.D. Biochemistry, 2007, University of Wisconsin--Madison (under Peter Belshaw)
B.S. Chemistry, B.S. Crop Science, 2001, University of Illinois--Urbana-Champaign (under Jack Widholm)
Research: Chemical biology, DNA-encoded medicinal chemistry, Proteomics
Research in the Krusemark laboratory focuses on the discovery of functional small molecules using the power of in vitro evolution. DNA-programmed combinatorial chemistry (DPCC) enables the "translation" of DNA constructs into DNA conjugates with wholly synthetic small molecules, thus allowing Darwinian-like selections. DPCC allows the synthesis and functional interrogation of small molecule libraries of unprecedented size and complexity (presently up to 10^10 members).
The lab works of the further development of the DPCC technology towards the goal of a streamlined system for generating drug leads and biological tool compounds. We use a combination of chemical, engineering, and molecular biology methods. We are working to expand the synthetic toolbox available for builiding molecules on unprotected DNA to produce molecules with more drug-like properties. Our lab will develop tools to study the protein kinase C (PKC) family of kinases, which are involved in the signal transduction pathways of numerous and varied cellular processes.
Lab Members:Kyle Edward Denton (Graduate Student)
Sarah G. Elder (Graduate Student)
Rachael R. Jetson (Post-Doctoral Research Associate)
Dongwook Kim (Research Scientist)
MCMP 205 Organic Chemistry II
Jim and Diann Robbers Cancer Research Grant (02/01/2014-01/31/2015)
(PI: Krusemark CJ)
In vitro evolution of substrates for highly specific kinase inhibition
Ralph W. and Grace M. Showalter Research Trust (07/01/14-06/30/15)
(PI: Krusemark CJ)
DNA-encoding to Enable Discovery of Active Small Molecules
Krusemark, C. J., Tilmans, N. P., Brown, P. O., Harbury, P. B., "Quantitative Population Behavior in Chemical Evolution." In review.
Tilmans, N. P., Krusemark, C. J., Harbury, P. B., “Expedient Synthesis of a Modular Phosphate Affinity Reagent.” Bioconj. Chem. 2010, 21, 1010-1013.
Frey, B. L., Ladror, D. T., Sondalle, S. B., Krusemark, C. J., Jue, A. L., Coon, J. J., Smith, L. M., “Chemical derivatization of peptide carboxyl groups for highly efficient electron transfer dissociation." J. Am. Soc. Mass Spectrom. 2013, 24, 1710-1721.
Krusemark, C. J., Frey, B. L., Belshaw, P. B., Smith, L. M., "Modifying the Charge State Distribution of Proteins in Electrospray Ionization Mass Spectrometry by Chemical Derivatization." J. Am. Soc. Mass Spectrom. 2009, 20, 1617-1625.
Krusemark, C. J., Ferguson, J. T., Wenger, C. D., Kelleher, N. L., Belshaw, P. J., "Global Amine and Acid Functional Group Modification of Proteins." Analytical Chemistry. 2008, 80, 713-720.
Krusemark, C. J. and Belshaw, P. J., "Covalent Labelling of Fusion Proteins in Live Cells via an Engineered Receptor-Ligand Pair." Organic and Biomolecular Chemistry. 2007, 5, 2201-2204.
Krusemark, C. J.*, Lamos, S. M.*, McGee, C. J., Scalf, M., Smith, L. M., Belshaw, P. J., "Mixed Isotope Photoaffinity Reagents for Identification of Small Molecule Targets by Mass Spectrometry." Angewandte Chemie Int. Ed. 2006, 45, 4329-4333. *Authors contributed equally.
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This record was last updated on Aug 21, 2015 at 3:43 PM